Use the QX200 droplet reader to count PCR-positive and PCR-negative droplets with an optical detector and thereby complete the second step of the Droplet Digital PCR (ddPCR™) workflow. Droplets are sipped and the singulator unpacks the emulsified droplets and streams them in single file past a two-color optical detection system in a serial manner.
QX200 Droplet Generator — utilizes proprietary reagents and microfluidics to partition samples into ~20,000 nanoliter-sized droplets QX200 Droplet Reader — following PCR amplification of the nucleic acid target in the droplets, this instrument analyzes each droplet individually using a two-color detection system (set to detect FAM and either VIC, HEX, or EvaGreen ®…
The QX200 droplet generator partitions samples into 20,000 nanoliter-sized droplets and, after PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 droplet reader. PCR-positive and PCR- negative droplets are counted to provide absolute quantification of.
The QX200 Droplet Generator partitions samples into ~20,000 nanoliter-sized droplets and, after PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 Droplet Reader. PCR-positive and PCR- negative droplets are counted to provide absolute quantification of.
Droplets were then subjected to thermal cycling with a Veriti Thermal Cycler (Thermo Fisher Scientific) (Additional file : Table S2), before transferring to a QX200 droplet reader (Bio-Rad) for fluorescence measurement of 6-fluorescein amidite (FAM) and hexachloro-fluorescein (HEX) probes. .. Droplets were scored as positive or negative based on their fluorescence intensity, which was determined by gating …
